7. DETECTION OF VIRULENCE FACTOR ASSOCIATED GENES OF CAMPYLOBACTER SPP. FROM CATTLE IN ANAND, GUJARAT, INDIA by DESAI, N. D., NAYAK, J. B., BRAHMBHATT, M. N.3, PATEL, S. N. AND KAJE, V. S.
Abstract
The aim of this study was to detect the virulence factor associated genes of Campylobacter spp. from cattle faecal samples in Anand city, Gujarat, India. Campylobacter isolates (n=8) used in this study were isolated from 200 faecal samples collected from the Anand city, Polymerase chain reaction technique was used for detection of five genes, namely flaA, cadF, iamA, flgR and cdtB genes of Campylobacter spp. Out of eight isolates, all the eight were positive for cadF, cdtB and flgR (100 percent) while six isolates were positive for flaA gene and four isolates were positive for iamA gene of Campylobacter spp. Presence of cadF, cdtB and flgR gene in all isolates shows that they are the specific targets for Campylobacter identification and are capable of producing gastroenteric illness to humans.
KEY WORDS: Campylobacter, Cattle, Gastroenteritis, Virulence genes.
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