1.ISOLATION AND PCR AMPLIFICATION OF GENOMIC DNA FROM LEAF SAMPLE OF GLORIOSA SUPERBA –A CONSERVATION APPROACH By K.V. RAVAL1_ B. N. PATEL1 AND B. K. JAIN2

Authors

  • K.V. RAVAL, B. N. PATEL AND B. K. JAIN

Abstract

Efficient method for isolation of high quality genomic DNA is the first step in the development of DNA based markers for genetic diversity study. Present study described a simple and efficient method for isolation of genomic DNA from leaves of G. superba. The modified method yields good quality, high molecular weight DNA, which is free from contaminants and coloured pigments. It is consistently amenable to PCR amplification and restriction digestion. This makes its wide use in various molecular biology applications i.e. genetic diversity study of the plant - as a conservation approach.
KEY WORDS: G. superba, Genomic DNA, PCR, Amplification, Genetic diversity, Conservation.

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Author Biography

K.V. RAVAL, B. N. PATEL AND B. K. JAIN

ISOLATION AND PCR AMPLIFICATION OF GENOMIC DNA FROM LEAF SAMPLE OF GLORIOSA SUPERBA –
A CONSERVATION APPROACH
K.V. RAVAL, B. N. PATEL AND B. K. JAIN
1. BIOTECHNOLOGY DEPARTMENT,
MEHSANA URBAN INSTITUTE OF SCIENCES,
GANPAT UNIVERSITY, GUJARAT, INDIA.
2. M. G. SCIENCE INSTITUTE, AHMEDABAD, GUJARAT, INDIA.
Corresponding author’s e-mail: ravalkalpeshkumar@yahoo.co.in, patel_sb@yahoo.com

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Published

2014-06-01

How to Cite

AND B. K. JAIN, K. R. B. N. P. (2014). 1.ISOLATION AND PCR AMPLIFICATION OF GENOMIC DNA FROM LEAF SAMPLE OF GLORIOSA SUPERBA –A CONSERVATION APPROACH By K.V. RAVAL1_ B. N. PATEL1 AND B. K. JAIN2. LIFE SCIENCES LEAFLETS, 52, 1 to 9. Retrieved from https://petsd.org/ojs/index.php/lifesciencesleaflets/article/view/679

Issue

Vol. 52 (2014): June, 2014.....ISSN 0976 - 1098(On Line) ISSN 2277 - 4297(Print)

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Articles

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