6.ISOLATION OF BACILLUS SPP. FROM SOIL FOR ANTIMICROBIAL PRODUCTION AND ANTIBIOTIC RESISTANCE By ASHISH KUMAR SHUKLA1_ RAVINDRA SINGH1_ PUSHPENDRA SINGH1_ PARAS GUPTA2 AND ARVIND KUMAR SRIVASTAVA
Abstract
Members of the Bacillus genus are generally found in soil and most of these bacteria have the ability to disintegrate proteins, namely proteolytic activity. Protease enzymes not only have important industrial uses, but also the proteases of these microorganisms play an important role in the nitrogen cycle, which contributes to the fertility of the soil. Bacillus spp. show higher level of resistance in supernatant and methanol. Ethyl acetate showed more effective against E. coli MTCC-1687.The result of this study showed that antimicrobial agent resistance was present in almost all isolates of Bacillus spp. isolated from different sample of soil. Isolates show higher level of resistance or reduced susceptibility to, Ampicillin and Ceftriaxone as there was less effective zone of inhibition observed. This study showed the distribution of antimicrobial agent resistance in Bacillus spp. isolates from a variety of sample and analysis of such pattern of resistance may prove to be useful beyond simple description.
KEY WORD: Isolation of bacillus, Antibiotic Resistance, Antimicrobial Production, Ampicillin, Ceftriaxone.
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