11.MICROBIAL DIVERSITY OF ALKALINE PHOSPHATASE PRODUCERS FROM LAKE ECOSYSTEM IN NORTH GUJARAT REGION_ INDIA By F. R. PATEL AND M. C. SHARMA

Authors

  • F. R. PATEL M. C. SHARMA

Abstract

Biodiversity of Alkaline phosphatase producing microbes in soil samples from water bodies
and lake ecosystems of North Gujarat region was studied. 57 types of microbes were isolated
through enrichment. High alkaline phosphatase producers were screened on Methyl Green
dye and Phenolphthalein diphosphate supplemented MG-PDP / Modified Dermatophyte test
agar (MDTA) media. Primary characterization of the isolates was carried out. 32 bacteria and
2 fungi produced alkaline phosphatase (ALP). The best six ALP producing bacteria were
further characterized by morphological-biochemical tests. All the 6 bacteria produced ALP
and other hydrolytic enzymes. ALP production was significant in liquid medium in the
alkaline pH range. ALP production was induced by organophosphorous insecticide Acephate
in Bacillus sp. FPB17 which can detoxify and mineralize a xenobiotic environmental
contaminant like organophosphate. Incorporation of PDP and Methyl Green indicator dye
can be utilized in the detection of ALP production.
KEY WORDS: Lake ecosystem; Alkaline phosphatase; Organophosphorous insecticide;
Methyl Green; Phenolphthalein diphosphate

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Author Biography

F. R. PATEL M. C. SHARMA

MICROBIAL DIVERSITY OF ALKALINE PHOSPHATASE
PRODUCERS FROM LAKE ECOSYSTEM IN NORTH
GUJARAT REGION, INDIA
F. R. PATEL * AND M. C. SHARMA
DEPARTMENT OF BIOTECHNOLOGY,
KADI SARVA VISHWAVISHVIDYALAYA, GANDHINAGAR-382023,
GUJARAT, INDIA.
fal2910@yahoo.co.in

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Published

2012-03-01

How to Cite

M. C. SHARMA, F. R. P. (2012). 11.MICROBIAL DIVERSITY OF ALKALINE PHOSPHATASE PRODUCERS FROM LAKE ECOSYSTEM IN NORTH GUJARAT REGION_ INDIA By F. R. PATEL AND M. C. SHARMA. LIFE SCIENCES LEAFLETS, 25, 87 to 98. Retrieved from https://petsd.org/ojs/index.php/lifesciencesleaflets/article/view/287

Issue

Vol. 25 (2012): MARCH, 2012.....ISSN 0976 - 1098(ON LINE) ISSN 2277 - 4297(PRINT)

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Articles

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