ISOLATION AND PURIFICATION OF GALLIC ACID, CAFFIEC ACID AND FERULIC ACID USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY FROM CLITORIA TERNATEA L.
Keywords:
polyphenols, caffiec acid, ferulic acid, gallic acid, clitoria ternateaAbstract
An effective, simple and easy HPLC methodology was developed for isolation and purification of caffiec acid, ferulic acid and gallic acid from fresh leaves of Clitoria ternatea L. These polyphenolic acids were separated using thin layer chromatography and to detect the purification Enable C-18 column were used in a binary gradient programme. Linear regression was analyzed for limit of detection and limit of quantification values. The method was optimized and standardized for quick isolation and purification for polyphenolic acids and will be useful for characterization of Clitoria ternatea L. collected from of the Lower Gangetic Plain from Hoogly (West Bengal, India).Downloads
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