5.MOLECULAR RESPONSES OF RICE (ORYZA SATIVA L.) TO ZINC STRESS BY A. JOHN DE BRITTO, M. SUTHA AND T. LEON STEPHAN RAJ.
Abstract
Seed germination is a complex physiological process in plants that can be affected severely by
stresses especially of heavy metals. In order to document the interference during germination by
Zinc stress at proteomic and genomic level, an investigation has been undertaken. Seeds were
exposed to different concentration of Zinc (0.10 and 0.15 ppm). Protein profiles analyzed by
two-dimensional electrophoresis (2-DE) revealed that proteins were differentially expressed in
Zinc-treated plants. Restriction digestion banding pattern of EcoRI and Hind III enzymes showed
distinct banding pattern in the treated plants compared to the control.
KEY WORDS: Heavy metal stress, Oryza sativa L., SDS- PAGE - polypeptides, Restriction
enzymes.
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