1. REDISCRIPTION OF CHRYSOMYA RUFIFACIES (MACQUART 1843) FROM OSMANABAD DISTRICT (MS) INDIA USING DNA BARCODING AND SCANNING ELECTRON MICROSCOPY by S.A. BANSODE AND V.R. MORE
In forensic entomology insect evidences and the developmental stages of the insects are used to determine the post-mortem interval (PMI). A PMI estimation is based either on insect developmental rates or on insect colonization and succession patterns of carrion. Correct identification is very important step in forensic entomology to determine correct PMI. Inaccurate identification may lead to inaccurate PMI. Lack of taxonomist to identify the species as well as the lack of sufficient keys, lead to occasional errors in the species identification. Usually the species identification from the maggots on gross examination is very difficult. The eggs or larvae of many forensically important dipteran species are particularly difficult to distinguish morphologically and an incorrect identification can seriously harm an investigation. To overcome the limitations of morphology-based identification, DNA based sequence data applied to identify insect species. Using molecular techniques morphologically similar species or genera can be distinguished easily. Scanning electron microscopy was performed to observe the body size, shape of wrinkled head, number of papillae, anterior, dorsal and posterior spines.