8. APPLICATION OF NEXT GENERATION SEQUENCING IN ANIMAL GENETICS by AMIYA RANJAN SAHU1, NIBEDITA NAYAK2, ARTH CHAUDHARI3, RAJESH KUMAR SAHU4 AND MAHENDRAKUMAR D. MAKWANA5
Abstract
Next generation sequencing is the procedure for identifying the precise order of nucleotides within a DNA molecule. It provides base that have been used for standard sequencing applications viz., genome sequencing and resequencing and also for novel applications. Generation leads to the chemistry and technology used by the sequencing process. Different methods of sequencing are available and it is applying in development of animal genetics. Next generation sequencing in animal genetics is applying for transcriptome sequencing, analysis of epigenetic modifications of histones and DNA, genomic selection, identification of all mutation in an organism at genomic level, nucleotide variation profiling, selection of resistant animal to disease, metagenomics, whole-exome sequencing (WES), single cell genomics, large-scale identification and development of molecular markers. Finally, it is concluded that Next Generation Sequencing has a broad applicability in functional genomics.
KEY WORD: Next Generation Sequencing, Animal Genetics, Genomic selection.
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