13. COMPARISON OF FRESH SEMEN PARAMETERS WITH FROZEN THAWED SEMEN FOLLOWING INCORPORATION OF TREHALOSE by K. Q. SHAIKH, H. C. NAKHASHI1, B. N. SUTHAR2, P.T SUTARIA3 AND V.K.SHARMA4

Authors

  • K. Q. SHAIKH, H. C. NAKHASHI B. N. SUTHAR, P.T SUTARIA AND V.K.SHARMA

Abstract

The research work was conducted to study comparison of fresh Kankrej bull’s semen parameters with frozen thawed semen following incorporation of different Trehalose concentrations, on the semen samples collected from three Kankrej bulls through a period of 12 weeks. The collected semen samples were pooled and examined for fresh semen and oxidative stress parameters and then diluted with Tris-based extender containing different Trehalose concentrations viz. 50mM, 100mM, 150mM and control, and evaluated for semen and oxidative stress parameters at frozen thawed stage of cryopreservation. Results clearly indicated that, cryopreservation led to a significant (P<0.05) decrease in frozen semen parameters in comparison with fresh semen. Therefore, 100mM Trehalose incorporated group showed significantly (P<0.05) better values in comparison to that of fresh semen, 50mM Trehalose, 150mM Trehalose and control groups, respectively. Howover, the sperm abnormality was significantly (P<0.05) lower in fresh semen and frozen thawed 100mM Trehalose semen, when compared to other and the control groups, respectively. Biochemical assays showed that, Malondialdehyde (MDA)
level was significantly (P<0.05) higher in fresh semen in comparison to that of frozen thawed semen
and Glutathione reductase (GSH) level was significantly (P<0.05) lower in fresh semen in
comparison to that of frozen thawed semen. Conclusively, supplementation of 100mM Trehalose in
the Tris-based extender is best concentration for cryopreservation of Kankrej bull’s semen.
KEY WORD: Trehalose, Kankrej bull, Oxidative stress, Malondialdehyde, Glutathione reductase.

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Author Biography

K. Q. SHAIKH, H. C. NAKHASHI B. N. SUTHAR, P.T SUTARIA AND V.K.SHARMA

COMPARISON OF FRESH SEMEN PARAMETERS WITH FROZEN THAWED SEMEN FOLLOWING INCORPORATION OF TREHALOSE K. Q. SHAIKH, H. C. NAKHASHI, B. N. SUTHAR, P.T SUTARIA AND V.K.SHARMA, 1, 2 & 4 DEPARTMENT OF GYNAECOLOGY AND OBSTETRICS, 3DEPARTMENT OF SURGERY & RADIOLOGY. COLLEGE OF VETERINARY SCIENCE AND ANIMAL HUSBANDRY, SARDARKRUSHINAGAR DANTIWADA AGRICULTURAL UNIVERSITY, NORTH GUJARAT-385506 INDIA. Corresponding author’s e-mail: shaikh.kashif786@gmail.com

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Published

2016-05-01

How to Cite

B. N. SUTHAR, P.T SUTARIA AND V.K.SHARMA, K. Q. S. H. C. N. (2016). 13. COMPARISON OF FRESH SEMEN PARAMETERS WITH FROZEN THAWED SEMEN FOLLOWING INCORPORATION OF TREHALOSE by K. Q. SHAIKH, H. C. NAKHASHI1, B. N. SUTHAR2, P.T SUTARIA3 AND V.K.SHARMA4. LIFE SCIENCES LEAFLETS, 76, 107 to 115. Retrieved from https://petsd.org/ojs/index.php/lifesciencesleaflets/article/view/1068

Issue

Vol. 76 (2016): JUNE, 2016.....ISSN 0976 - 1098(On Line) ISSN 2277 - 4297(Print)

Section

Research Paper

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