4. HEPATOPROTECTIVE ACTIVITY OF BALIOSPERMUM AXILLARE ROOT ON EXPERIMENTAL LIVER INJURY IN RATS by DURGA K. MEWARA1, RUCHI SINGH1, KUMUD TANWAR1, MRIDULA SHARMA1,M.C. SHARMA1 AND R.S. GUPTA2
The present experimental study was to evaluate the methanol extract of root of B. axillare for hepatoprotective activity against carbon tetrachloride-induced hepatotoxicity in experimental rats. B. axillare root extract exhibited significant (P<0.001) hepatoprotective activity by reducing carbon tetrachloride-induced change in biochemical parameters that was evident by antioxidants and enzymatic examinations. The plant root extract may interfere with free radical formation, oxidative stress and lipid peroxidation, which may conclude as hepatoprotective agent. The results were comparable with the standard drug silymarin. From this extract, the active constituents-stigmasterol, ceryl alcohol, octacosanol-1, b-sitosterol, betulin, betulinic acid and lupeol were isolated by IR, 1H NMR, 13C NMR and MS studies. Chronic toxicological studies revealed that the LD50 value is more than the dose of 3g/kg body weight.