2. STUDY OF CHLOROPHYLL VARIATION “A” AND “B” FROM SELECTED AQUATIC PLANTS by Dr. A.J. PARMAR
Chlorophyll a, b, c, d, Carotene, Xanthophylls are present in all higher plants. Then chlorophyll a and b are most important for plants. Make extract of chlorophyll in 80% acetone and estimate by spectrophotometer in Optical Density. Chlorophyll a and b are measured in amount of mg/mL. Chlorophyll is estimate of hydrophytes plants in Ammannia baccifera L., Eichhornia crassipes (Mart.) Solms, Hydrilla verticillata (L.f.)Royle, Nymphaea pubescens Willd, Bacopa monnieri (L.) Wettst. and Typha angustifolia L. The range of chlorophyll contains are 1.9826 to 6.9220 mg/mL for chlorophyll a and 2.6598 to 10.7774 mg/mL for chlorophyll b. Concentration of chlorophyll a and b was calculated using Arnon method. Chlorophyll content was higher in Nymphaea pubescens than other Aquatic plants.