12. DEVELOPMENT OF A NOVEL, COST EFFECTIVE, VARIABLE FREEZING RATE APPARATUS FOR CRYOPRESERVATION OF FISH SEMEN FOR FIELD USE by RAVINDRAGOUDA PATIL1, WAZIR SINGH LAKRA2, SHRINIVAS JAHAGEERDAR2, GOPAL KRISHNA2 AND ASI

Authors

  • RAVINDRAGOUDA PATIL, WAZIR SINGH LAKRA, GOPAL KRISHNA AND ASIM KUMAR PAL

Abstract

In the present study, a variable freezing rate apparatus with a novel design was fabricated using cost effective materials and tools for the cryopreservation of the spermatozoa of threatened mahseer fish species belonging to the genus Tor. The apparatus consists of a rack stand and straw racks made of aluminium flat strips. The straw rack has a provision to hold about 42 straws. The main advantage of the apparatus is that, the three racks can be simultaneously frozen at the same or at three different freezing rates. The rack stand with straw-loaded straw racks can be easily kept into a styrofoam box with liquid nitrogen and frozen at desired freezing rates. This apparatus was successfully used for the cryopreservation of the endangered cold water fish species of the Deccan mahseer, Tor khudree and the Golden mahseer, T. putitora.
KEY WORDS: Cost effective, Freezing apparatus, Straw rack, Rack stand, Cryopreservation, Mahseer.

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Author Biography

RAVINDRAGOUDA PATIL, WAZIR SINGH LAKRA, GOPAL KRISHNA AND ASIM KUMAR PAL

DEVELOPMENT OF A NOVEL, COST EFFECTIVE, VARIABLE FREEZING RATE APPARATUS FOR CRYOPRESERVATION OF FISH SEMEN FOR FIELD USE
RAVINDRAGOUDA PATIL, WAZIR SINGH LAKRA, SHRINIVAS JAHAGEERDAR, GOPAL KRISHNA AND ASIM KUMAR PAL
1CURRENT AFFILIATION: FISHERIES RESEARCH AND INFORMATION CENTER, KVAFSU, HESSARAGHATTA, BENGALURU, KARNATAKA STATE, INDIA.
2FISH GENETICS AND BIOTECHNOLOGY DIVISION, CENTRAL INSTITUTE OF FISHERIES EDUCATION, VERSOVA, MUMBAI- 400 061, MAHARASHTRA STATE, INDIA.
Corresponding author’s e-mail: : ravi.patil30@gmail.com

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Published

2016-07-01

How to Cite

GOPAL KRISHNA AND ASIM KUMAR PAL, R. P. W. S. L. (2016). 12. DEVELOPMENT OF A NOVEL, COST EFFECTIVE, VARIABLE FREEZING RATE APPARATUS FOR CRYOPRESERVATION OF FISH SEMEN FOR FIELD USE by RAVINDRAGOUDA PATIL1, WAZIR SINGH LAKRA2, SHRINIVAS JAHAGEERDAR2, GOPAL KRISHNA2 AND ASI. LIFE SCIENCES LEAFLETS, 77, 113 to 117. Retrieved from https://petsd.org/ojs/index.php/lifesciencesleaflets/article/view/1082

Issue

Vol. 77 (2016): JULY, 2016.....ISSN 0976 - 1098(On Line) ISSN 2277 - 4297(Print)

Section

Research Paper

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