MONITORING AND ANALYSIS OF PETROL BIODEGRADATION USING CHROMATOGRAPHIC METHODS

  • A.H. JOBANPUTRA AND V. R. KOTHARI A.H. JOBANPUTRA AND V. R. KOTHARI DEPARTMENT OF MICROBIOLOGY, PSGVPM’S S. I. PATIL ARTS ,G. B. PATEL SCIENCE & S. T. S. K V. S.COMMERCE COLLEGE, SHAHADA – 425 409, MAHARASHTRA, INDIA. Corresponding author’s e-mail: arpana_jobanputra@rediffmail.com

Abstract

Due to wide spread use of petrol in situ biodegradation of pollutants has attracted immense attention in the few decades. Oil contaminated soil isolates, identified as Pseudomonas aeruginosa and Acinetobacter lwoffii  by 16S ribosomal DNA (rDNA) sequencing and phylogentic anaylsis were used for the present investigation. The bacterium grew optimally at 370C and pH 6.5 and maximum growth was obtained at 5% (v/v) petrol concentration. The intermediate of biodegradation catechol was detected by HPLC. Biodegradation of petrol was monitored by quantitative gas chromatographic analysis. The percent degradation of petrol by Pseudomonas aeruginosa Pseudomonas aeruginosa and  Acinetobacter lwoffiiAcinetobacter lowffi Acinetobacter lwoffii Acinetobacter lwoffi&&77&77&&7&&&&&&&&&&&&&&&&&&&7&7&7&&&77andaaa within 25 days  was found to be 95% and 97% respectively. These strains were thereby suggestive for application in petrol biodegradations under all investigated nutritional and environmental conditions in polluted soil.

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Published
2014-03-01
How to Cite
V. R. KOTHARI, A.H. JOBANPUTRA AND. MONITORING AND ANALYSIS OF PETROL BIODEGRADATION USING CHROMATOGRAPHIC METHODS. LIFE SCIENCES LEAFLETS, [S.l.], v. 49, mar. 2014. ISSN 0976-1098. Available at: <http://petsd.org/ojs/index.php/lifesciencesleaflets/article/view/46>. Date accessed: 21 july 2017.