IDENTIFYING GENETIC LOCUS ASSOCIATED WITH BROWN PLANTHOPPER (NILAPARVATA LUGENS (STAL.)) RESISTANCE INVOLVING BULKED SEGREGANT ANALYSIS IN RICE (ORYZA SATIVA L.)

  • G. PAVITHRADEVI*, S. ROBIN AND M. MAHESW G. PAVITHRADEVI*, S. ROBIN AND M. MAHESWARAN CENTRE FOR PLANT BREEDING AND GENETICS, TNAU, COIMBATORE, TAMIL NADU. Corresponding author’s e-mail: pavigurusamy @gmail.com

Abstract

The brown planthopper (BPH), Nilaparvata lugens (Stål) (Homoptera: Delphacidae), is one of the major insect pests causing severe damage to the rice crop. Several major genes and Quantitative Trait Loci (QTL) associated with BPH resistance in rice were identified and put into high yielding varieties of rice. In the present study, an attempt has been made to identify the genetic locus responsible for resistance to BPH involving 264 F2 individuals from the cross between IR50 (susceptible to BPH) and Bharathi (resistant to BPH). The parental survey revealed 50.12% polymorphism between parents. Bulked Segregant Analysis method was adopted to identify the polymorphic markers associated with susceptibility/ resistance. Theses markers were surveyed on F2 individuals to establish linkage/ association with resistance to BPH following linkage analysis. The association of putative markers identified based on DNA pooling from selected segregants was confirmed by linkage analysis using MAPMAKER/EXP 3.0. The results of linkage analysis revealed that SSR marker, RM6487 on chromosome 4 showed significant association with resistance to BPH.

 

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Published
2017-04-30
How to Cite
M. MAHESW, G. PAVITHRADEVI*, S. ROBIN AND. IDENTIFYING GENETIC LOCUS ASSOCIATED WITH BROWN PLANTHOPPER (NILAPARVATA LUGENS (STAL.)) RESISTANCE INVOLVING BULKED SEGREGANT ANALYSIS IN RICE (ORYZA SATIVA L.). LIFE SCIENCES LEAFLETS, [S.l.], v. 87, apr. 2017. ISSN 0976-1098. Available at: <http://petsd.org/ojs/index.php/lifesciencesleaflets/article/view/377>. Date accessed: 21 july 2017.

Keywords

Rice, Brown planthopper, Modified Seed-box Screening Test, Bulked segregant analysis.