SODIUM FLUORIDE ALTERATION OF PROTEIN CONTENT VIS-À-VIS ELECTROPHORETIC PATTERN OF MUSCLE ESTERASES (E.C.3.1.1.1) IN POECILIA RETICULATA PETERS ON CHRONIC EXPOSURE

  • HITESH U. SHINGADIA AND **E.R. AGHARIA HITESH U. SHINGADIA AND **E.R. AGHARIA *DEPT. OF ZOOLOGY; **DEPT. OF CHEMISTRY SVKM’S MITHIBAI COLLEGE OF ARTS, CHAUHAN INSTITUTE OF SCIENCE & AMRUTBEN JIVANLAL COLLEGE OF COMMERCE & ECONOMICS, VILE PARLE (WEST) MUMBAI 400 056 MAHARASHTRA, INDIA. Corresponding author’s e-mail: hiteshshingadia26@gmail.com

Abstract

ABSTRACT:

A progressive reduction in protein content observed in the muscle of fish in present study was both as a function of time as well as increase in the concentration of fluoride. During chronic exposure to sodium fluoride, the banding pattern of esterase diminished in the treated group of fish viz.  lowest (5.75 ppm), lower intermediate (7.18 ppm), higher intermediate (9.58 ppm) and highest (14.37 ppm) concentration of the 24 hrs. LC50 (115 ppm) value when compared with the control group. SDS-PAGE and staining of the gel revealed that esterase in muscle of fish from control group resolved into six bands (lane-1). Exposure of fluoride to all the four concentrations showed significantly faint and diffused banding pattern of esterases and complete loss of esterase band-1 (Lane 2-5), probably due to chronic stress induced by fluoride. The esterase from band-1 might be sensitive to fluoride intoxication, thus completely vanished during chronic treatment. However in the higher intermediate (9.58 ppm) and highest (14.37 ppm) sodium fluoride treatment groups, sixth band of esterase (lane 4-5) was found to be very faintly visible on staining. Decrement in protein content & diminution of certain esterase bands in the muscle tissue of the treated group suggest soft tissue (non-skeletal) fluorosis induced by sodium fluoride during chronic exposure period, probably could be due to inhibition of biosynthetic mechanism of proteins vis-à-vis esterases. The study of esterase in fish calls attention to sensitive indicator of the environmental pollutants and can be used as contrivance in study of environmental dilapidations.

KEY WORDS: Protein, Esterase isozymes, Electrophoresis, Sodium fluoride, Poecilia reticulata.

References

REFERENCES:
Achyutha Devi J. and Piska Ravishankar (2006). Effect of fluoride on tissue proteins of freshwater catfish, Clarius batrachus (Linn). J. Aqua. Biol. Col. 21(1): 184-187.
Ahmed M.J., Alam M.S., Rashid M.A., Begum R.A., Shahjahan R.M. (2011). Variability of esterase isozyme at some developmental stages of mosquito fish, Poecilia reticulata. Bangladesh J. Life Sci. 23(1): 139-142.
Amita Saxena and Ningthoukhongjam Soranganba (2012). Esterases diversity in Indian and exotic carps. 1(1): 24-28.
Begum R.A., Yasmin F., Rashid M.A., Alam M.S., Shahjahan R.M. (2011). Comparison of tissue specific esterase isozyme banding pattern in the larvae and adult of Heteropneustes fossilis. Indian J. Soc. Nat. Sci. 1: 1-7.
Chinoy N.J., Joseph R, Sequeira E. and Narayan M.V. (1991): Effects of sodium fluoride on the muscle and liver of albino rats. Ind. J. Environ. Toxicol. 1(2): 129-134.
Choudhary A. and Gaur Shalini (2001). Effect of sodium fluoride on the muscle and liver of freshwater fish, Cyprinus carpio. J. Aqua. Biol. 16(2): 67-68.
E.R. Agharia (2014). Groundwater quality assessment in some villages of Vadgam Taluka of Banaskantha District, Gujarat, India, with an emphasis on study of fluoride and its effects. International J. of Chemistry. 5: 16-31.
Ferguson M.M. and Danzmann R.G. (1998). Role of Genetic Markers in Fisheries and Aquaculture: Useful Tools or Stamps Collection. Can. J. Fish. Aquat. Sci. 55:1553-1563.
Hitesh U. Shingadia (2012), “Sodium fluoride induced changes in embryonic protein profile of Guppy, Poecilia reticulata Peters”. Bionano Frontiers 5(1): 158-160.
Hongtuo F., Zin D., Tingting W. (1993). Isozyme of Oreochromis aureus. Aquaculture. 11:326-332.
Knowles C., Aruvkar S.K., Hogan J.W. (1968). Electrophoretic separation of fish brain esterase. J. Fish. Res. Bd. Can. 25:121-129.
Laemmli U.K. (1970): Nature, 227: 680.
Lowry O.H., Rosebrough N.J., Farr A.L. and Randall R.J. (1951). J. Biol. Chem. 193:265.
Nelson J.S., Cox C.S. (1993). Principles of Biochemistry, third edition, CBS publishers, Delhi, India: 125-175.
P.C. Mishra and Khageshwar Pradhan (2007). Prevalence of Fluorosis among school children and cattle population of Hirakud Town in Orissa. The Bioscan. 2(1): 31-36.
Padhi B.K. and Mandal R.K. (1995). Restriction Site Polymorphism in Mitochondrial DNA of Indian Major Carps. Curr. Sci. 68 (9):965-967. www.sciencejournal.in 1(1): 2012.
Richardson B.J. (1986). Geographical distribution of electrophoretically detected protein variation in Australian commercial fishes. III. Western king prawn, Penaeus latisulcatus Kishinouye. Australian J. Mar. Fresh Res. 33(5): 933–937.
Shahjahan R.M., Karim A. Begum R.A., Alam M.S. and Begam A. (2008). Tissue specific esterase isozyme banding pattern in Nile Tilapis (Oreochromis niloticus). Univ. J. Zool. Rajshahi Univ. 27: 1-5.
Shaklee J.B. and Kenan C.P. (1986). A practical laboratory guide to technique and methodology of electrophoresis and its application in fish fillet identification, Report 177, CSIRP, Australia, Marine Laboratories.519.
Shaklee J.B., Phelps S.R. and Salini J. (1990). Analysis of fish stock structure and mixed stock fisheries by electrophoretic characterization of allelic isozyme. In: Whitmore, D.H. ed. Electrophoretic and isoelectric focusing techniques in fisheries Management. CRC press. Bocaraton, Florida.173-196.
Shingadia Hitesh and Sakthivel Veena (2008). “Impact of short-term exposure of Sodium fluoride on tissue biochemistry of Guppy, Poecilia reticulata Peters”, J. Aqua. Biol. 22(2): 148-152.
Sifa L., Wangi C., Biyun Z. (1993). Variation of morphology and biochemical genetic markers among population of blunt snout bream (Megalobrana mblycephala). Aquaculture 1993; 111: 117-125.
Utter F.M. and Folmer L. (1978). Protein systems in grass carp: allelic variability and relationship in Pacific salmon and related trout based on protein variations. Syst. Zool. 22: 257-270.
Ward R.D. (2000). Genetics in Fisheries Management. Hydrobiol. 420:191-201.
Zhu J. Indrasith L.S. & Yamashita O. (1986). Biochem. Biophys. Acta. 882:427.
How to Cite
**E.R. AGHARIA, HITESH U. SHINGADIA AND. SODIUM FLUORIDE ALTERATION OF PROTEIN CONTENT VIS-À-VIS ELECTROPHORETIC PATTERN OF MUSCLE ESTERASES (E.C.3.1.1.1) IN POECILIA RETICULATA PETERS ON CHRONIC EXPOSURE. LIFE SCIENCES LEAFLETS, [S.l.], v. 59, dec. 2014. ISSN 0976-1098. Available at: <http://petsd.org/ojs/index.php/lifesciencesleaflets/article/view/206>. Date accessed: 24 july 2017.

Keywords

Protein, Esterase isozymes, Electrophoresis, Sodium fluoride, Poecilia reticulata.