FIXATION OF LETHAL DOSE AND EFFECT OF ETHYL METHANE SULFONATE INDUCED MUTAGENESIS IN RICE ADT(R) 47

  • D.RAJARAJAN1, R.SARASWATHI1, D. SASSIKUMAR 2 AND S.K GANESH3 D.RAJARAJAN1, R.SARASWATHI1, D. SASSIKUMAR 2 AND S.K GANESH3 1 PLANT BREEDING AND GENETICS UNIT, TAMIL NADU RICE RESEARCH INSTITUTE (TNAU), ADUTHURAI, TAMIL NADU, INDIA. 2 SOIL AND WATER MANAGEMENT RESEARCH INSTITUTE (TNAU), THANJAVUR, TAMIL NADU, INDIA. 3DEPARTMENT OF PLANT BREEDING AND GENETICS, AD AGRICULTURAL COLLEGE AND RESEARCH INSTITUTE, (TNAU), TRICHY, TAMIL NADU, INDIA. Corresponding author’s e-mail: rajarajandeva@gmail.com

Abstract

ABSTRACT:

A study was conducted to determine the lethal dose of chemical mutagen EMS in rice cultivar ADT (R) 47. Genetically pure seeds were treated with different concentrations of EMS viz., 80mM, 100mM, 120mM, 140mM and 160mM. Untreated seeds soaked in distilled water was used as check for comparison. The LD50 values were observed based on growth reduction of seedlings after EMS treatment. The LD50 dose for EMS under invitro and invivo condition was fixed at 126mM and 121mM based on probit analysis. As the concentration of applied EMS increased, there was a decrease in germination, survival rate of seedlings, root length, shoot length, seedling height, vigour index under invitro conditions and emergence and survival under field (invivo) conditions in M1 generation as compared to the control. The survival percentage of seedlings was higher at higher doses of EMS under invivo conditions compared to invitro conditions.

References

REFERENCES:
Acquaah, G. 2007. “Principles of Plant Genetics and Breeding,” Blackwell Publishing Ltd., Oxford.
Auerbach, C and Robson. J. M. 1946. “Chemical Production of Mutations,” Nature, Vol. 157. No. 3984, p. 302.
Bhat, R, Upadhyaya. N, Chaudhury. A, Raghavan. C, Qiu. F, Wang. H, Wu. J, McNally. K, Leung. H and Till. B. 2007. “Chemical-and Irradiation-Induced Mutants and Tilling,” In: Upadhyaya N.M, Ed., Rice Functional Genomics: Challenges, Progress and Prospects, Springer, New York, pp. 148-180.
Finney, D. J. 1971. Probit analysis. Cambridge University Press.
Finney, D. J. 1978. Statistical method in biological assay. Charles Griffin and Co.
Hajra, N.G. 1979. Induction of mutations by chemical mutagens in tall indica rice. Indian Agric., 23: 67–72
Harding, S. S, Johnson. S. D, Taylor. D. R, Dixon. C. A and Turay. M. Y. 2012. Effect of Gamma Rays on Seed Germination, Seedling Height, Survival Percentage and Tiller Production in Some Rice Varieties Cultivated in Sierra Leone. American Journal of Experimental Agriculture, 2(2): 247-255.
Minocha, J. L and Arnason. T. J. 1962. Mutagenic effectiveness of ethyl methane Sulfonate in barley. Nature, 196: 499
Singh. S and Singh. J. 2003. Mutation in basmati rice induced by gamma rays, ethyl methane Sulfonate and sodium azide. Oryza, 40(1&2): 5-10.
Talebi, A.B, Talebi, A.B, and Shahrokhifar. B. 2012. Ethyl Methane Sulfonate (EMS) Induced Mutagenesis in Malaysian Rice (cv. MR219) for Lethal Dose Determination. American Journal of Plant Sciences, 3: 1661-1665.
How to Cite
D. SASSIKUMAR 2 AND S.K GANESH3, D.RAJARAJAN1, R.SARASWATHI1,. FIXATION OF LETHAL DOSE AND EFFECT OF ETHYL METHANE SULFONATE INDUCED MUTAGENESIS IN RICE ADT(R) 47. LIFE SCIENCES LEAFLETS, [S.l.], v. 58, nov. 2014. ISSN 0976-1098. Available at: <http://petsd.org/ojs/index.php/lifesciencesleaflets/article/view/191>. Date accessed: 21 sep. 2017.

Keywords

Rice, Ethyl Methane Sulfonate (EMS), Lethal Dose 50 (LD 50).