STANDARDIZING THE EXTRACTION AND EVALUATION OF ANTIMICROBIAL FRACTION FROM STREPTOMYCES SP. TC1 AGAINST XANTHOMONAS ORYZAE PV. ORYZAE

  • NANJUNDAN JAIVEL*, RAMASAMY RAJESH AND PONNUSAMY MARIMUTHU DEPARTMENT OF AGRICULTURAL MICROBIOLOGY, TAMIL NADU AGRICULTURAL UNIVERSITY, COIMBATORE, TAMIL NADU, INDIA. Corresponding author’s e-mail: jaivelmicro@gmail.com
Keywords: Streptomyces, Xanthomonas oryzae pv. oryzae, antimicrobial, MIC.

Abstract

Streptomyces are prokaryotic microorganisms capable of producing a wide range of secondary metabolites has the capacity to fight with the competing plant pathogens and suppress the growth of them. A promising Streptomyces sp. TC1 isolate exhibited antimicrobial activity against bacterial leaf blight pathogen Xanthomonas oryzae pv. oryzae under in vitro conditions. The antimicrobial activity of TC1 culture filtrate was found to be higher at seven days of incubation. The optimized extraction of antimicrobial fractions from the culture filtrate was obtained with ethyl acetate solvent. Adjusting the pH of the culture filtrate to 3 prior to extraction process resulted in increased recovery of antimicrobial fractions. The crude antimicrobial fraction obtained from the TC1 fermentation broth exhibited a minimum inhibitory concentration value of 300 µg/ml against the test pathogen Xanthomonas oryzae pv. oryzae. The antimicrobial activity results including agar well diffusion assay and MIC experiments strongly recommend that the Streptomyces sp. TC1 can be explored for antimicrobial metabolites for bacterial blight disease control in rice.

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Published
2014-08-01