3. ISOLATION OF PHOSPHATE SOLUBILIZING BACTERIA FROM RHIZOSPHERE OF BT COTTON PLANT GROWN IN MEHSANA DISTRICT, NORTH GUJARAT by PARMAR, H. B.,1 RAOL, B.V.2 AND ACHARYA, P.B.1
Phosphorus is one of the major nutrients which play an indispensable biochemical role in photosynthesis, respiration, energy storage and transfer, cell division, cell enlargement and several other processes in the living plant. Phosphate solubilizing bacteria (PSB) can solubilize different forms of inorganic phosphates. A total of twenty nine (29) phosphate solubilizing bacteria were isolated from the six (06) rhizospheric sample of BT cotton plant of Mehsana District, Gujarat. Out of 29 isolates; 14 isolates show remarkable zone of solubilization. The zone of solubilization was studied on pikovskaya’s agar and quantitative phosphate solubilization was carried out by vanado- molybdate method. Burkholderia latens showed the maximum phosphate solubilization index 4.27 ± 0.094 in PVK agar plates along with phosphate solubilizing activity 328.7 ± 7.13 μg mL-1 in PVK broth and pH of the medium decreased upto 4.14 ± 0.067. However, the isolate PSB-3 shows the least solubilizing activity. Potent isolates show good phosphate solubilizing ability and thus were found potential further used as biofertilizers agents.
KEY WORD: Phosphate Solubilizing bacteria, Burkholderia latens, Bt Cotton, Rhizosphere.