ISOLATION AND PCR AMPLIFICATION OF GENOMIC DNA FROM LEAF SAMPLE OF GLORIOSA SUPERBA – A CONSERVATION APPROACH

  • K.V. RAVAL1, B. N. PATEL1 AND B. K. JAIN2 1. BIOTECHNOLOGY DEPARTMENT, MEHSANA URBAN INSTITUTE OF SCIENCES, GANPAT UNIVERSITY, GUJARAT, INDIA. 2. M. G. SCIENCE INSTITUTE, AHMEDABAD, GUJARAT, INDIA. Corresponding author’s e-mail: ravalkalpeshkumar@yahoo.co.in, patel_sb@yahoo.com

Abstract

Efficient method for isolation of high quality genomic DNA is the first step in the development of DNA based markers for genetic diversity study. Present study described a simple and efficient method for isolation of genomic DNA from leaves of G. superba. The modified method yields good quality, high molecular weight DNA, which is free from contaminants and coloured pigments. It is consistently amenable to PCR amplification and restriction digestion. This makes its wide use in various molecular biology applications i.e. genetic diversity study of the plant - as a conservation approach.

 

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How to Cite
B. K. JAIN2, K.V. RAVAL1, B. N. PATEL1 AND. ISOLATION AND PCR AMPLIFICATION OF GENOMIC DNA FROM LEAF SAMPLE OF GLORIOSA SUPERBA – A CONSERVATION APPROACH. LIFE SCIENCES LEAFLETS, [S.l.], v. 52, may 2014. ISSN 0976-1098. Available at: <http://petsd.org/ojs/index.php/lifesciencesleaflets/article/view/101>. Date accessed: 18 oct. 2017.

Keywords

G. superba, Genomic DNA, PCR, Amplification, Genetic diversity, Conservation.