EFFECT OF PH AND TEMPERATURE ON THE ACTIVITY AND STABILITY OF LIPASE ISOLATED FROM TRICHODERMA HARZIANUM by SIVA KESAVARAO KOMMULA1, JAYAKUMAR KANNALI2, PRASAD UNDRAJAVARAPU3 & KRISHNA SOWJANYA KANCHANA4
The main aim of this study is to investigate the effect of pH and temperature on the activity and stability of lipase isolated from Trichoderma harzianum. The pH-relative activity (%) profile of lipase activity was determined by using p-NPB as a substrate and the optimum pH was found to be 8.5. The T. harzianum lipase was extremely stable at an optimal pH and retained at least 70% of its original activity at a pH range of 8.0- 9.0, after 24 h of incubation at 4Â°C. . The optimum temperature of T. harzianum lipase was found to be 40 Â°C and it was observed that enzyme preparation had very high activity at temperatures ranging from 30 to 50 Â°C.
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