EFFECT OF PH AND TEMPERATURE ON THE ACTIVITY AND STABILITY OF LIPASE ISOLATED FROM TRICHODERMA HARZIANUM by SIVA KESAVARAO KOMMULA1, JAYAKUMAR KANNALI2, PRASAD UNDRAJAVARAPU3 & KRISHNA SOWJANYA KANCHANA4

  • SIVA KESAVARAO KOMMULA1
Keywords: Lipase, Trichoderma harzianum, Optimal pH, Optimum temperature.

Abstract

The main aim of this study is to investigate the effect of pH and temperature on the activity and stability of lipase isolated from Trichoderma harzianum. The pH-relative activity (%) profile of lipase activity was determined by using p-NPB as a substrate and the optimum pH was found to be 8.5. The T. harzianum lipase was extremely stable at an optimal pH and retained at least 70% of its original activity at a pH range of 8.0- 9.0, after 24 h of incubation at 4°C. . The optimum temperature of T. harzianum lipase was found to be 40 °C and it was observed that enzyme preparation had very high activity at temperatures ranging from 30 to 50 °C.

Author Biography

SIVA KESAVARAO KOMMULA1

SIVA KESAVARAO KOMMULA1, JAYAKUMAR  KANNALI2,  PRASAD UNDRAJAVARAPU3  & KRISHNA SOWJANYA KANCHANA4

1DEPARTMENT OF BIOCHEMISTRY, DR Y.S.R. HORTICULTURAL UNIVERSITY, ANANTHARAJUPETA, ANDHRA PRADESH, INDIA.

2DEPARTMENT OF MICROBIOLOGY, S.V. UNIVERSITY TIRUPATHI.

3DEPARTMENT OF BIOTECHNOLOGY, ACHARYA NAGARJUNA UNIVERSITY, GUNTUR, ANDHRA PRADESH, INDIA.

 4DEPARTMENT OF BIOTECHNOLOGY, ANDHRA UNIVERSITY, VISAKHAPATNAM, ANDHRA PRADESH, INDIA.

Corresponding author email:  kskraok @gmail.com

References

1. Ayhan, Z., Yeom, H.Y., Zhang, Q.H. and Min, D.B. (2001). Flavor, color, and vitamin C Cardenas J, Alvarez E, de Castro-Alvarez M-S, Sanchez-Montero J-M, Valmaseda M, Elson SW, Sinisterra J-V. Screening and catalytic activity in organic synthesis of novel fungal and yeast lipases. J Mol Catal B: Enzym 2001;14:111 – 23.
2. Costa MA, Peralta RM. Production of lipase by soil fungi and partial characterization of lipase from a selected strain (Penicillium wortmanii). J Basic Microbiol 1999;39:11 – 5.
3. Dharmsthiti S, Ammaranond P. Purification and characterization of lipase from a raw-milk yeast (Tricho-sporon asteroides). Biotechnol Appl Biochem 1997;26:111 – 6.
4. Dong H, Gao S, Han SP, Cao SG. Purification and characterization of a Pseudomonas sp. lipase and its properties in non-aqueous media. Biotechnol Appl Biochem 1999;30:251 – 6.
5. Ducret A, Trani M, Lortie R. Lipase catalysed enantioselective esterification of ibuprofen in organic solvent under controlled water activity. Enzyme Microb Technol 1998;22:212 – 6.
6. Elibol M, Ozer D. Influence of oxygen transfer on lipase production by Rhizopus arrhizus. Process Biochem 2001;36:325 – 9.
7. El-Shafei HA, Rezkallah LA. Production, purification and characterization of Bacillus lipase. Microbiol Res 1997;52:199 – 208.
8. Guit RPM, Kloosterman M, Mindersma GW, Mayer M, Meijer EM. Lipase kinetics: hydrolysis of triacetin by lipase from Candida cylindracea in a hollow fiber membrane reactor. Biotechnol Bioeng 1991;38:727 – 32.
9. Hou CT. pH dependence and thermostability of lipases from cultures from ARS culture collection. J Ind Microbiol 1994;13:242 – 8.
10. Ishihara H, Okumura H, Ikezawa H, Tejima S. Studies on lipase from Mucor javanicus. Biochim Biophys Acta 1975;388:413 – 7.
11. Jaeger KE, Reetz TM. Microbial lipases from versatile tools for biotechnology. Trends Biotechnol 1998;16: 396 – 403.
12. Janssen EM, Sjurenes JB, Vakurov AV, Halling PJ. Kinetics of lipase-catalyzed esterification in organic media: correct model and solvent effects on parameters. Enzyme Microb Technol 1999;24:463 – 70.
13. Kouker G, Jaeger KE. Specific and sensitive plate assay for bacterial lipases. Appl Environ Microbiol 1987;53:211 – 3.
14. Wang Y, Srivastava KC, Shen GJ, Wang HY. Thermostable alkaline lipase from a newly isolated thermophilic Bacillus strain, A30-1 (ATCC 53841). J Ferment Bioeng 1995;79:433 – 8.
15. Wilke D. Chemicals from biotechnology: molecular plant genetics will challenge the chemical and fermentation industry. Appl Microbiol Biotechnol 1999;52:135 – 45.
16. Yeoh HH, Wong FM, Lin G. Screening for fungal lipases using chromogenic lipid substrates. Mycologia 1986;78:298 – 300.
Published
2017-06-27