1. COMPARATIVE STUDY OF CHEMICAL VARIANTS IN IN VITRO AND WILD GROWN ROOT EXTRACTS OF VALLERIANA WALLICHI DC. by ABIGAIL H ZIMOMI1, KALPANA KUKREJA1, RONALD DEBBARMA2, NEELAM KHARE2 AND SANDEEP ROUT2
The laboratory experiment was carried out during May-August 2013 at Department of Forestry, in Doon (PG) College of Agriculture Science and Technology, Dehradun (Uttarakhand), for comparative study on varied phyto-chemicals present among different extracts of in vitro and wild grown Valeriana wallichi. Extract yield per cent was found highest with WDW (25%). Phytochemical screening of Valeriana extracts indicated the presence of different classes of secondary metabolites. Proteins, amino acids, flavonoids, betacyanins, tannins, coumarins, terpenoids and saponins were present in all the tested extracts i.e IM, WM, IDW and WDW. Phenols, anthocyanins and carbohydrates are present only in in vitro extracts (IM and IDW). However, glycosides, quinones, and, gums and mucilages were absent. Flavonoids were analyzed quantitatively in Valeriana extracts. Flavonoid content was found higher in in vitro root extracts (IM and IDW) as compared to wild root extracts (WM and WDW). The results revealed that IDW extract has highest (51.7 mg/g) flavonoid and WM has lowest flavonoid content i.e. 13.5 mg/g. The analysis of phytochemicals was also performed using the gas chromatography-mass spectroscopy (GC-MS) method. GC-MS chromatogram showed that total of 110 and 126 chemical compounds were identified with IM and WM respectively. Total of thirteen major compounds were found in in vitro and wild grown root extracts. Valepotriate i.e. Isovaleric acid (WM - 3.12 % and IM - 1.56%) and valerenic acid (IM - 7.74% and WM 6.56%) were observed. Another compound, valerana was seen in very small amount (IM - 0.36% and WM - 0.20%). Viridiflorol: WM (15.41%) and IM (3.45%) were found. However, viridiflorene was present only in WM (0.36%) extract. Among fatty acids, palmitic acid was seen in IM - 11.64% and WM - 2.35%.